NIAB - National Institute of Agricultural Botany

Assay information: HYPTE3SNP

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Narrative This KASP assay belongs to a set of 67 validated assays designed to recapitulate polymorphisms targeted by SNPshot or CAPS assays as described in Ellwood et al, BMC Genomics (2008). Further details of the validation of this set of KASP assays can be found in Cottage et al, Mol Breeding (2012) and the related online supplementary data. The assay has been integrated into a consensus map with 653 new markers. Details in Webb et al.,Plant Biotechnology Journal (2015).
References Cottage A, Gostkiewicz K, Thomas JE, Borrows R, Torres A-M, and O Sullivan D.M. (2012) Heterozygosity and diversity analysis using mapped SNPs in a faba bean inbreeding programme. Molecular Breeding 30 (4). pp. 1799-1809. ISSN 1572-9788 doi: 10.1007/s11032-012-9745-4 . Ellwood S, Phan H, Jordan M, Hane J, Torres A, Avila C, Cruz-Izquierdo S, Oliver R (2008) Construction of a comparative genetic map in faba bean (Vicia faba L.); conservation of genome structure with Lens culinaris. BMC Genetics 9:380.
Map 2015 consensus map
Linkage Group Vf_Chr_6 B
Chromosome unknown
cM position 128.32
Assay ID 972-0041
Assay Name HYPTE3SNP
Reference Allele Sequences
Sequence ID Allele Phenotype
FH893745 T NA
FH893876 A NA
Polymorphism sequence ATCTGCTCTGTTGTTGCACCAAGCAGATTGGCTTTTATGGCTTCTTCATGGAAAG
CTTGGAGTTTCAGATTATAATAATGCTCTGAAGGCATCATTTAATAAGCTCTAAC
TTATTATCTGTTTTATAGCTATAGGGTAAATCATAATTATGTCTGTTGCGATATC
CGAGTTGGAAAAT[W]TATCGATTGTTTTTATGTTTTGCAGGTTGGGTATGATCC
TGAAGCTAACTCATATCCATCATGGCTAATCTCTC
Reference allele sequence alignment
Validation plot
Genotype data
Accession Name Other Ref Genotype call (with filter) Reference sample of known genotype
Verde_Bonita T:A
VF27 T:T
ig11749-5/S1 A:A
ig11903-1/S2 T:T
ig11903-1/S3 T:T
ig12137-2/S2 T:T
ig12137-2/S3 T:T
ig11276-1/S1 T:T
ig12159-1/S1 T:T
ig12159-1/S2 T:T
ig12263-1/S1 T:T
ig12263-1/S2 T:T
ig12613-2/S2 T:T
ig12613-2/S3 T:T
ig12658-1/S1 T:T
ig12658-1DW/S2 T:T
ig12747-1/S1 T:T
ig12747-2DW/S1 T:T
ig12747-2/S2 T:T
ig13004-1/S1 T:T
ig11290-1/S1 T:T
ig11290-1/S2 T:T
ig14096-5/S1 T:A
ig14189-1/S2 T:T
ig14189-1/S3 T:T
ig14197-2/S1 T:T
ig14197-2/S2 T:T
ig70718-5/S1 T:A
ig72355-5/S1 T:A
ig72423-1/S1 A:A
ig72423-1/S2 A:A
ig72495-1/S2 T:T
ig124126-1/S1 A:A
ig124126-1/S2 A:A
ig124126-2/S1 A:A
ig124126-2/S2 A:A
ig124213-3/S1 T:T
ig124300-1/S1 T:T
ig124301-1/S1 T:T
ig11531-5/S1 T:T
ig130596-2/S1 T:T
ig130596-2/S2 T:T
ig130638-1/S1 T:T
ig130674-1/S1 T:T
ig130734-2/S1 T:T
ig131693-1/S1 T:T
ig131693-1/S2 T:T
ig132660-2/S2 T:T
ig132813-2/S1 A:A
Borington Bulk-1 T:T
Borington Bulk-1/S1 T:T
V219-4/S1 T:T
V220-3/S1 T:T
NA1-2/S1 T:T
NA12-1/S1 T:T
Vf172-1 T:T
Vf172-1/S1 T:T
GLV45-2/S1 T:T
Hedin/2-1 T:T
Hedin/2-1/S1 T:T
Maris Bead-3 T:T
Maris Bead-3/S1 T:T
Fuego-4 T:T
Fuego-4/S1 T:T
Granit-1 T:T
Granit-1/S1 T:T
Albus-1 T:T
Kasztelan-1 T:T
ig101760-3 (BPL1) T:T
ig101760-3/S1 (BPL1) T:T
ig101769-1/S1 (BPL10) T:T
ig101769-1/S2 (BPL10) T:T
ig101770-1/S1 (BPL11) ?
ig101770-1/S2 (BPL11) T:T
ig101771-4/S1B1 (BPL12) T:T
ig101771-3 (BPL12) T:T
ig101780-3/S1 (BPL21) T:T
ig101782-3 (BPL23) T:T
ig101782-3/S1 (BPL23) T:T
ig101786-2/S1 (BPL27) T:T
ig101786-2/S2 (BPL27) T:T
ig101799-1/S1 (BPL40) T:T
ig101799-1/S3 (BPL40) T:T
ig101822-2/S1 T:T
ig101822-2/S2 (BPL63) T:T
ig101942-3 (BPL183) T:T
ig101942-3/S1 (BPL183) T:T
CGN07715 cf-3-2 (60354-9) T:T
CGN07715 cf-3-2 (60354-9)-1/S1A1 T:T
FAB4000104-13-1 T:A
FAB4000104-13-1/S1 T:A
Vf6-1/S1 A:A
Vf136-1/S1 T:T
INRA29H-1/S1 A:A
ig11656-6/S1 T:A
ig11687-7/S1 T:T
ig11695-5/S1 T:T
NV153_1DW_S3A2 T:T
NV639_1_HEDIN T:T
NV643_3_S2 T:T
NV644_1_KASZTEL T:T
NV648_2_S3B2_7 T:T
NV656_3_S2A2_9 T:T
NV657_26 A:A
NV658_2_CGN0771 T:T
NV662_1_VF136 T:T
NV713_1_COTEDOR A:A
NV714_1_HIVERNA A:A
NV715_1_WEBO A:A
NV716_1_WIBO A:A
NV717_1_L79_79 A:A
NV718_1_L977_88 T:T
NV719_1_L979_S1 T:T
NV720_1_BOURDON T:T
NV721_1_ARRISOT T:T
NV722_1_BANNER T:T
NV723_1_BULLDOG T:T
NV724_1_PIETRAL T:T
NV725_1_GIZA3_2 T:T
NV726_1_GIZA402 A:A
NV727_1_BPL4628 T:T
NV728_1_TW T:T
NV729_1_VF6 A:A
NV730_1_I4347_4 A:A
NV731_1_I4347_3 A:A
NV732_1_BPL710 T:T
NV733_1_NA112 T:T
NV734_1_ILB938 T:T
NV735_1_MELODIE A:A
NV736_1_AURORA T:T